Eggs oocytes are retrieved from patients by transvaginal aspiration directly from the ovarian follicles, approximately 36 hours after an injection of HCG. At that time, the follicular fluid is taken into the laboratory so that the eggs can be identified by the embryologists As oocytes mature within the developing follicle, they are surrounded by cumulus cells which participate in the nourishment of the egg. The egg and the cumulus are referred to as the oocyte-cumulus complex. The dishes containing the eggs are then placed into an incubator, so that the environmental conditions surrounding the eggs can be tightly controlled with regard to light, oxygen and carbon dioxide concentrations, as well as the pH and temperature. The cumulus cells are left attached to the oocyte for at least 4 hours post-retrieval to allow the oocytes to complete their maturation. The assessment of fertilization takes place by evaluating each egg the day after the oocyte retrieval and looking for the presence of two pronuclei.

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He told her not to, as the oil is good for the skin. She then told me that it is my turn, and only then I remembered that I had asked planned on getting a massage as well. Of course, my wife now wanted to join in the massage, and I tried to get out of it, as I do not enjoy massages as much as my wife. But she insisted, and Sridhar seemed to be keen on it also, so I gave in.